Complete Guide to Understanding Single-Cell RNA-Seq - Active …
2021年3月4日 · Single-Cell RNA-Seq techniques have made it possible to study transcriptomics in heterogeneous samples; driving advances in our understanding of cancer, embryonic development and neurodegenerative disease. This article covers the history, protocols, and applications of Single-Cell RNA-Seq.
Single‐cell RNA sequencing technologies and applications: A …
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art approach for unravelling the heterogeneity and complexity of RNA transcripts within individual cells, as well as revealing the composition of different cell types and functions within highly organized tissues/organs/organisms.
A practical guide to single-cell RNA-sequencing for biomedical …
2017年8月18日 · In this review, we present a practical guide to help researchers design their first scRNA-seq studies, including introductory information on experimental hardware, protocol choice, quality control, data analysis and biological interpretation.
Single-cell RNA sequencing technologies and bioinformatics …
2018年8月7日 · Single-cell RNA sequencing (scRNA-seq), for example, can reveal complex and rare cell populations, uncover regulatory relationships between genes, and track the trajectories of distinct cell ...
Applications of single-cell RNA sequencing in drug discovery …
2023年4月28日 · Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods, together with associated computational tools and the growing availability of public data resources, are...
The Evolution of Single-Cell RNA Sequencing Technology and …
As an emerging sequencing technology, single-cell RNA sequencing (scRNA-Seq) has become a powerful tool for describing cell subpopulation classification and cell heterogeneity by achieving high-throughput and multidimensional analysis of individual cells and circumventing the shortcomings of traditional sequencing for detecting the average trans...
Introduction to Single-Cell RNA-seq Data Analysis
2024年9月3日 · We explain the fundamental principles of single-cell sequencing, the detailed methodology, and its diverse applications in fields. Focusing on single-cell RNA sequencing (scRNA-seq), we highlight its significance in capturing the transcriptomic profiles of individual cells and advancing our understanding of cellular functions and interactions.
Single-Cell RNA Sequencing Procedures and Data Analysis
2021年3月20日 · Single-cell and single-nuclei sequencing experiments reveal previously unseen molecular details. The number of sequencing procedures and computational data analysis approaches have been increasing rapidly in recent years. This chapter provides an overview of the current developments in single-cell analysis.
An Introduction to the Analysis of Single-Cell RNA-Sequencing …
Single-cell RNA sequencing has been uniquely valuable to gain insights into cellular heterogeneity in tissues and for identification of previously unknown cell types. 1, 2, 3 Single-cell technologies can also be used to define subpopulations within a known cell type by searching for differential gene expression patterns within the cell populatio...
Single-cell RNA sequencing: Technical advancements and …
2018年2月1日 · Single-cell transcriptomic atlases provide unprecedented resolution to reveal complex cellular events and deepen our understanding of biological systems. In this review, we summarize and compare single-cell RNA sequencing technologies, that were developed since 2009, to facilitate a well-informed choice of method.