TUNEL Assay: A Powerful Tool for Kidney Injury Evaluation
2021年1月2日 · We will discuss aspects of TUNEL as it applies to kidney diseases and injury and identify qualities, techniques, and underused features of TUNEL to promote the productive and appropriate use of this powerful assay in research, diagnostics, and therapy of kidney diseases.
Polynucleotide phosphorylase protects against renal tubular injury …
2023年3月3日 · Analysis of tubular atrophic tissues from renal dysfunction patients and male mice with ischemia-reperfusion injuries (IRI) or unilateral ureteral obstruction (UUO) treatment shows that renal...
TUNEL Assay: A Powerful Tool for Kidney Injury Evaluation
2021年1月2日 · Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3'-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quanti ….
TUNEL Apoptotic Cell Detection in Tissue Sections: Critical …
TUNEL, i.e., terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling, has become a widely used staining method to assist in detection of apoptotic cells in tissue sections.
TUNEL Assay: A Powerful Tool for Kidney Injury Evaluation
2021年1月2日 · Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA...
TUNEL Assays | Thermo Fisher Scientific - US
Thermo Fisher Scientific has developed a modified TUNEL assay that incorporates an alkyne-modified dUTP, EdUTP (Figure 1) at the 3'-OH ends of fragmented DNA using terminal deoxynucleotidyl transferase (TdT).
Characterization of ferroptosis in kidney tubular cell death under ...
2021年2月8日 · TUNEL-positive tubular cells were identified in formalin-fixed kidney tissue by examining at least 15 regions at ×20 magnification. Data are expressed as the number of positively stained...
Analysis of apoptosis of kidney tissue by the tunel method and ...
2020年11月1日 · In this study, it was aimed to investigate whether there is a significant difference in the number of apoptotic cells in partial obstruction (PO) and complete obstruction (CO) by using terminal deoxytransferase-mediated bio-dUTP nick and labeling (TUNEL) method in renal tissue.
TUNEL Assay: A Powerful Tool for Kidney Injury Evaluation
2021年1月1日 · Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation by endonucleases and is becoming increasingly popular for kidney injury assessment because it can be used universally in cultured and tissue cells and for all mechanisms of cell death.
The miR-26a-5p/IL-6 axis alleviates sepsis-induced acute kidney …
TUNEL assay TUNEL staining was performed to identify apoptotic cells in renal tissues using a reagent (12156792910) from Roche Life Science. Briefly, tissue sections were deparaffinized and pretreated with 0.1 M sodium citrate (pH 6.0) at 65 °C for 30 min and then incubated with a TUNEL reaction mixture for 1 h at 37 °C in a humidified, dark ...