Visualize Microtubule Dynamics in Live Cells
To visualize microtubule dynamics in live cells, Thermo Fisher Scientific has developed Invitrogen Tubulin Tracker Deep Red, a fluorescent molecule that selectively stains microtubules in live cells. Eliminating the need for genetic modification, this product provides uniform labeling of the microtubule cytoskeleton in live cells with a rapid ...
CellLight™ Tubulin-GFP, BacMam 2.0 - Thermo Fisher Scientific
Image tubulin dynamics in live cells with CellLight Tubulin-GFP. This ready-to-use fluorescent protein-based reagent contains a tubulin targeting sequence fused to emerald GFP (emGFP) and utilizes the powerful BacMam delivery technology.
Probes for Tubulin and Other Cytoskeletal Proteins—Section 11.2
Label tubulin in live or fixed cells using Molecular Probes fluorescent reagents, including paclitaxel conjugates, antibodies, and tubulin-targeted GFP and RFP.
Fluorogenic probes for live-cell imaging of the cytoskeleton
2014年5月25日 · We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells.
Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of individual cytoskeletal proteins and any antibodies to be used; background fluorescence.
Fluorescent dyes and probes for super-resolution microscopy of ...
2018年3月28日 · We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents – taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components.
Simple and efficient delivery of cell-impermeable organic fluorescent ...
2019年8月14日 · Numerous commercial organic fluorophores with excellent optical properties are precluded from live-cell superresolution imaging due to poor cell permeability. Here, we develop a simple but...
Cytosolic delivery of cell-impermeable fluorescent probes ... - Cell …
2023年11月15日 · Here, we develop a general strategy to deliver cell-impermeable organic fluorescent probes, including various SNAP/Halo tag probes, into live cells with excellent delivery efficiency (up to 97% labeling) and almost 100% endo/lysosomal release by simple coincubation with a novel type of peptide vehicle, mixLPV, a mixture of two cell-penetrating p...
Fluorescent Tubulin Proteins - Cytoskeleton
We continually strive to provide the purest, most biological active and relevant tubulin proteins, kits and reagents for today's researchers. The following fluorescent tubulin proteins are useful for monitoring microtubule dynamics in living cells .
Fluorescent Live Cell Imaging of Cytoskeleton ... - MilliporeSigma
Imaging of microtubule dynamics in live cells expressing tubulin tagged with fluorescent proteins has contributed greatly to our understanding of microtubule-binding agents. We utilized LentiBrite™ lentiviral biosensors to visualize microtubules (α-tubulin), actin microfilaments (β-actin), microfilament cross-link sites (α-actinin ...