NEBridge Golden Gate
NEBridge Golden Gate Assembly Tool can be used to design primers for Golden Gate DNA Assembly reactions, estimate overhang fidelity, or find optimal Golden Gate break points for long sequences.
NEBridge® Golden Gate Assembly | NEB
NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly. For additional flexibility and convenience for users, NEBridge ® Ligase Master Mix performs high efficiency and high-fidelity Golden Gate Assembly with a broad assortment of NEB Type IIS restriction enzymes.
Golden Gate Assembly Tool Tutorial - NEB
This video demonstrates how to use the Golden Gate Assembly Tool, including selecting inserts and plasmids, primer design and full construct sequence file.
Overview of NEBridge® Tools for Golden Gate Assembly | NEB
Learn to navigate NEBridge Golden Gate Assembly & Ligase Fidelity Tools for expert DNA design. In this video, we will introduce you to the multiple web tools that New England Biolabs offers to assist you with designing high-fidelity, high-efficiency Golden Gate Assemblies using NEB Type IIS restriction enzymes.
Golden Gate Assembly Workflow | NEB
Golden Gate Assembly is a method for assembling multiple DNA fragments, relying on the activity of Type IIS endonucleases that cleave outside of non-palindromic recognition sites. In this example, the destination vector is designed to include BsaI recognition sequences, in opposing directions, to allow for the assembly of the desired fragments.
Getting Started with Golden Gate Assembly | NEB
2024年12月16日 · In Golden Gate Assembly, a vector that fails to join with the insert is re-linearized by the restriction enzymes, reducing the background of empty vectors in the final product. Figure 2: Type IIP vs. Type IIS Restriction Enzymes . In summary, Golden Gate Assembly conceptually requires much of the same design logic as classic cloning:
Please use the free NEBridge Golden Gate Assembly Tool to design primers for your Golden Gate Assembly reactions. Use NEBridge Ligase Fidelity Tools to predict overhang fidelity or find optimal Golden Gate junctions for long sequences.
Golden Gate Assembly | NEB
Golden Gate Assembly Tool Tutorial This video demonstrates how to use the Golden Gate Assembly Tool, we will walk through selecting insert and plasmids, primer design to make amplicon inserts.
NEB - Golden Gate Assembly Protocol for using NEBridge Golden Gate …
2020年1月15日 · (4) The NEBiocalculator® Tool (nebiocalculator.neb.com) can be used for molar calculations. (5) For assemblies ≤ 10 inserts, use 1 μl ; for assemblies > 10 inserts, use 2 μl.
This assembly kit contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. BsaI-HFv2 has been engineered by NEB and outperforms BsaI in Golden Gate Assemblies. Together these enzymes can direct the assembly of multiple inserts/modules and also single insert/library generation cloning with single insert(s) using the Golden Gate approach.
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