小鼠免疫细胞标志物指南 | Cell Signaling Technology
浆细胞样 DC 共表达 Siglec-H 和 CD317，并产生 I 型 IFNγ，而常规 DC 的特点是表达 CD11c 和 MHCII。 擅长于向 CD8 + T 细胞进行交叉呈递的 DC 可通过 XCR1 或 CLEC9A 的表达来检测。
CD11b immunophenotyping identifies inflammatory profiles in the mouse ...
2015年9月30日 · In mice, lung innate immune cell alterations during endotoxin challenge, influenza virus infection, and in two genetic models of chronic obstructive lung disease could be segregated based on the...
Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
2020年6月19日 · Microglia are the macrophages in the brain and are easily identified as CD45 int CD11b + F4/80 + Ly6C − (Figure 3 A). In the epidermis, there are two main populations of immune cells, Langerhans cells (LCs) and dendritic epidermal T cells (DETCs). LCs are CD11b + F4/80 + and also express CD11c and MHCII (Figure 3 B).
Recommendations for myeloid-derived suppressor cell
2016年7月6日 · Early studies on inflammation in the mouse highlighted a shared, systemic expansion of myeloid cells bearing the markers CD11b (CR3A or integrin αM) and Gr-1 (anti-Gr-1 mAbs recognize epitopes...
Blood Monocytes Consist of Two Principal Subsets with Distinct ...
2003年7月1日 · Monocytes are defined as blood mononuclear cells with bean-shaped nuclei, expression of CD11b, CD11c, and CD14 in humans and CD11b and F4/80 in mice, and lack of B, T, NK, and DC markers.
CD11b immunophenotyping identifies inflammatory profiles in the mouse …
2016年3月1日 · Identification of distinct inflammatory profiles in mouse bronchoalveolar lavage (BAL). (a) CD11b vs. CD11c expression profiles of human alveolar macrophages (AMΦs) compared with healthy adult C57BL/6 mice. Human BAL samples collected and analyzed on separate days, with laser voltages standardized by the use of …
Mouse dendritic cells and other myeloid subtypes in healthy …
2022年8月9日 · Dermal cDC2 are characterized from the CD64 – Ly-6C – population by gating on CD11c + MHC-II + cells that express CD11b and CD172a (Fig. 2E). In addition, we investigated CD115, F4/80 and CD64 expression on the non-DC myeloid cell populations P1-P5 (Fig. 2F ).
CD11c + CD88 + CD317 + myeloid cells are critical mediators of ... - PNAS
We generated CD11c.Cre +/−ITGA4fl/fl C57BL/6 mice to selectively delete α4-integrin in CD11c + cells. Active immunization and adoptive transfer EAE models were employed and compared with WT controls. Multiparameter flow cytometry was utilized to immunophenotype leukocyte subsets.
Multi-parameter immune profiling of peripheral blood ... - Nature
2019年12月19日 · Using antibody Panel A, in which HLA-DR, CD19, CD44, CD4, CD11c, CD16, CD3, CD56, CD14, CD8a, T-bet, CD33, CCR2 and CD11b were used for meta-clustering, we detected nine distinct clusters and a...
Single-cell profiling of CD11c+ B cells in atherosclerosis
2024年1月8日 · The aged mouse model showed an expansion of CD11c+ B cells in the spleen, blood, and bone marrow, which was highly associated with atherosclerotic plaque burden. Additionally, CITESeq also identified DN2 and ABC subtypes of CD11c+ B cells to be associated with severe atherosclerosis in humans.