N-GSDMD trafficking to neutrophil organelles facilitates IL
2020年5月5日 · Gasdermin-D (GSDMD) in inflammasome-activated macrophages is cleaved by caspase-1 to generate N-GSDMD fragments. N-GSDMD then oligomerizes in the plasma …
GSDMD-dependent neutrophil extracellular traps promote
2022年8月8日 · In vitro, GSDMD-dependent NETs triggered p65 translocation to the nucleus, which boosted the production of inflammatory cytokines and α-SMA expression in …
TRIM21 regulates pyroptotic cell death by promoting …
2021年9月11日 · Here, we show that tripartite motif protein TRIM21 acts as a positive regulator of GSDMD-dependent pyroptosis. TRIM21 interacted with GSDMD via its PRY-SPRY domain, …
Control of gasdermin D oligomerization and pyroptosis by the …
2021年7月20日 · Flow cytometry demonstrated that the frequency of PI positive cells increased over time in a Dox-dependent manner for cells expressing NT-GSDMD (Figure 1F). Confocal …
Gasdermin D permeabilization of mitochondrial inner and outer …
2023年11月3日 · Here, we show that the N-terminal pore-forming GSDMD fragment (GSDMD-NT) rapidly damaged both inner and outer mitochondrial membranes (OMMs) leading to reduced …
The Pore-Forming Protein Gasdermin D Regulates ... - ScienceDirect
2018年1月16日 · Cell- and liposome-based assays demonstrated that GSDMD pores were required for IL-1β transport across an intact lipid bilayer. These findings identify a non …
Caspase-11/GSDMD contributes to the progression of ... - Springer
2024年3月4日 · In an effort to unravel the effects of Gsdmd on renal inflammation, we conducted immunofluorescence staining and flow cytometry analyses. Immunofluorescence staining …
Mitochondrial ROS promotes susceptibility to infection via …
2022年7月29日 · Using flow cytometry, we measured GSDMD mitochondrial association by co-staining isolated mitochondria with MitoTracker and anti-GSDMD antibodies after AIM2 …
GSDMD enhances cisplatin-induced apoptosis by promoting the …
2022年3月14日 · Flow cytometry showed that GSDMD overexpression resulted in a significant increase in cell death upon cisplatin treatment, and DMF did not attenuate the cell death …
Gasdermin D deficiency aborts myeloid calcium influx to drive ...
2024年6月3日 · Interestingly, RNA sequencing and flow cytometry revealed that myeloid GSDMD deficiency enhanced granulopoiesis at the hematopoietic sites in LN mice, exhibiting …