pAAV-HDC-DIO-GFP-shRNA.scramble - Addgene
Expresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression
Lateral hypothalamic proenkephalin neurons drive threat-induced ...
2023年10月28日 · At 24 h post-PSS exposure, we found that shRNA-mediated knockdown of Penk significantly reduced HFD consumption, but not NC intake, whereas PSS mice injected with control virus (AAV-DIO-eGFP ...
Improved AAV vector system for cell-type-specific RNA interference
2022年2月15日 · In summary, we developed a conditional, CRE-regulated AAV-DIO-based vector and demonstrated that it effectively initiates the simultaneous expression of shRNA and fluorescent protein in cells and tissues by targeting the TREK-1 gene.
TGR5-mediated lateral hypothalamus-dCA3-dorsolateral septum …
2024年3月21日 · To further determine role of Kv4.2 in antidepressant-like effects of TGR5, a Cre-dependent shRNA against Kv4.2 (AAV-DIO-Kv4.2shRNA-mCherry) was infused into LHA of susceptible GAD2-Cre mice (Figures 4I and 4J).
Distinct circuits and molecular targets of the paraventricular ...
2024年11月20日 · Accumulated evidence has confirmed that the paraventricular hypothalamus (PVH) plays an important role in the perception of visceral and somatic pain, whereas the exact neural pathways and molecules distinguishing them remain unclear.
Improved AAV vector system for cell-type-specific RNA interference
2022年2月15日 · We verified the Cre-dependent knockdown efficiency of the newly developed CREon shRNA vector in both HEK293T cells overexpressing TREK-1 and PC3 cells with endogenous TREK-1.
Cre-inducible Adeno-associated Virus: DIO-AAV – SignaGen Blog
2015年10月9日 · For cell type-specific targeting and to capitalize on the large number of Cre driver lines with the flexible virus injection/fiberoptic approach, we have developed a tool we call DIO-AAV (doublefloxed inverse orf) AAV.
AKAP150 from nucleus accumbens dopamine D1 and D2 receptor …
2023年11月17日 · Dopamine D1 receptor-expressing medium spiny neurons (D1R-MSNs) and dopamine D2 receptor-expressing MSNs (D2R-MSNs) in nucleus accumbens (NAc) have been demonstrated to show different effects on reward and memory of abstinence.
TRIM45 causes neuronal damage by aggravating microglia
2022年2月25日 · AAV2/6 viruses encoding U6-DIO-Scramble-EGFP and U6-DIO-TRIM45 shRNA-EGFP were injected into Cx3cr-Cre mice to induce microglia-specific TRIM45 knockdown.
An ultra-short-acting benzodiazepine in thalamic nucleus reuniens ...
2024年6月14日 · The AAV vector carrying Gabrg2-shRNA also featured a double-floxed inverted orientation (DIO) coding sequence for shRNA-resistant Gabrg2-EGFP (read as Gabrg2*), expressed exclusively in the presence of Cre recombinase (Fig. 4b).