Crystal Structure of the Complex of Human FasL and Its Decoy …
2016年11月1日 · To further investigate how FasL interacts with both DcR3 and Fas, we created a FasL mutant by replacing the C-terminal segment of the FasL AA′ loop (residues 164–169; sequence: DTYGIV) with the cognate region from TL1A (sequence: HELGLA).
Signaling and transcriptional control of Fas ligand gene expression
2003年3月25日 · The intracellular and extracellular domains of FasL are located in the N- and C-terminal regions, respectively (see Figure 1 for FasL structure).
Posttranslational regulation of Fas ligand function
2008年12月29日 · C-terminal to its transmembrane region (TM), a stretch comprising aa 137 to 183 in human FasL was shown to be essential for trimerisation and self-assembly of FasL (SA). The C-terminal moiety is characterised by its homology to other TNF ligand ectodomains (THD).
SPR sensorgrams of injections of purified DcR3 (C) or Fas (D) across immobilized E.coli derived wild type FasL at various concentrations. Saturation binding curves for each experiment are shown in the right panels. (E) Summary of the affinities of all FasLs with Fas or DcR3.
FasL structure and potential sites of posttranslational …
C-terminal to its transmembrane region (TM), a stretch comprising aa 137 to 183 in human FasL was shown to be essential for trimerisation and self-assembly of FasL (SA). The C-terminal...
Disruption of Fas-Fas Ligand Signaling, Apoptosis, and Innate …
2014年8月7日 · Experimentally, the contribution of FasL to inflammatory diseases can be assessed using C57BL/6 FasL gld mice, which contain a single residue mutation (F275L) within FasL that prevents binding to the Fas receptor .
Molecular mechanisms of FasL-mediated ‘reverse-signaling’
2020年11月1日 · The cytoplasmic tail of FasL possesses motifs that can recruit and initiate the activation of MAPKs. The C-terminal Class II SH3-binding site [PXXPX(R/K)] of FasL can interact with the SH3 domains of adaptor molecules Grb2, Gads, and Grap (Thornhill et al., 2008).
FasL Expression and Reverse Signalling | SpringerLink
2009年1月1日 · As indicated by the name-giving TNF homology domain (THD), the highest degree of homology between individual members of the TNF family is seen in the extracellular part of the type II transmembrane proteins, although the receptor-specific binding sites are located at the very C-terminus.
The C-terminal Domain of the Long Form of Cellular FLICE …
Thus, these results reveal that the C-terminal domain of c-FLIP L specifically inhibits the interaction between the caspase 8 prodomain and the RIP1 DD and, thereby, regulates caspase 8-dependent NF-κB activation in response to FasL stimulation.
FasL-Independent Activation of Fas - Madame Curie Bioscience …
Fas death receptor (also named CD95 or APO-1) is physiologically activated through binding to its cognate ligand, FasL. Fas/FasL interaction induces oligomerization and aggregation of Fas receptor, leading eventually to apoptosis after protein-protein interactions with adaptor and effector proteins.
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