Horseradish peroxidase - Wikipedia
The enzyme horseradish peroxidase (HRP), found in the roots of horseradish, is used extensively in biochemistry applications. It is a metalloenzyme with many isoforms, of which the most studied type is C. It catalyzes the oxidation of various organic substrates by hydrogen peroxide.
辣根过氧化物酶 - 百度百科
辣根过氧化物酶（Horseradish Peroxidase, HRP），是 临床检验 试剂中的常用酶。该产品不但广泛用于多个生化检测项目，也广泛运用于免疫类（ ELISA ）试剂盒。 过氧化物酶 作为多个试剂盒显色体系的关键成分，对试剂盒的质量有重要影响。
辣根过氧化物酶 - 维基百科，自由的百科全书
辣根过氧化物酶 （英語： horseradish peroxidase，简称为 HRP）存在于 辣根 中，在 生物化学 领域有着广泛地应用，主要是因为它具有将微弱信号放大及增强靶标分子可检测度的能力。 ^ PDB 1w4y; Carlsson GH, Nicholls P, Svistunenko D, Berglund GI, Hajdu J. Complexes of horseradish peroxidase with formate, acetate, and carbon monoxide. Biochemistry. January 2005, 44 (2): 635–42. PMID 15641789. doi:10.1021/bi0483211.
An updated view on horseradish peroxidases: recombinant …
Horseradish peroxidase (HRP) first appeared in scientific literature more than 200 years ago and has been the subject of numerous studies until now and ongoing. Scientific interest in HRP spiked in the late 1980s and early 1990s with the breakthrough of molecular diagnostics, molecular biology tool kits, and the publication of the first HRP ...
Horseradish peroxidase: a modern view of a classic enzyme
2004年2月1日 · Horseradish peroxidase is an important heme-containing enzyme that has been studied for more than a century. In recent years new information has become available on the three-dimensional structure of the enzyme and its catalytic intermediates, mechanisms of catalysis and the function of specific amino acid residues.
Horseradish Peroxidase - Chemistry LibreTexts
Horseradish peroxidase uses hydrogen peroxide to oxidize both organic and inorganic compounds. 1 Horseradish peroxidase along with other heme peroxidases are brightly colored especially under the near-ultraviolet light. 2 This property of heme peroxidases make them useful for attaching to “transparent” proteins so that they can be seen ...
Horseradish Peroxidase - an overview | ScienceDirect Topics
Horseradish peroxidase is an archetypal haem-containing enzyme that uses hydrogen peroxide (H 2 O 2) or alkyl peroxide to oxidize a variety of organic and inorganic compounds.
Horseradish Peroxidase - an overview | ScienceDirect Topics
Horseradish peroxidase (HRP) is an enzyme that catalyzes the oxidation of diaminobenzidine, forming an electron-dense product that can easily be identified in tissues fixed with osmium tetroxide for electron microscopy; FM1–43 is an amphipathic styryl dye that becomes highly fluorescent on partitioning into cell membranes. When frog muscles ...
Inactivation kinetics of horseradish peroxidase (HRP) by …
2023年8月17日 · In the present work, the effect of H 2 O 2 on the inactivation of horseradish peroxidase (HRP) was evaluated. Three states of HRP (E 0, E 2, and E 3) were identified. While in the absence of H 2...
The catalytic pathway of horseradish peroxidase at high resolution
2002年5月23日 · Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound...
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