Clostridium perfringens - microbewiki - Kenyon College
2011年4月22日 · Cell structure and metabolism. Clostridium perfringens are non-motile rod-shaped Gram-positive bacteria. It possesses the typical characteristics of Gram-positive bacteria, such as a protective thick cell wall, which is made up …
Clostridium perfringens Infection - StatPearls - NCBI Bookshelf
2023年8月8日 · Clostridium perfringens is an anaerobic gram-positive spore-forming bacillus associated with acute gastrointestinal infections ranging in severity from diarrhea to necrotizing enterocolitis and myonecrosis in humans. This pathogen possesses an arsenal of toxins responsible for disease pathogenesis and can form spores resistant to environmental ...
Clostridium perfringens Iota-Toxin: Structure and Function
Clostridium perfringens iota-toxin is composed of the enzyme component (Ia) and the binding component (Ib). Ib binds to receptor on targeted cells and translocates Ia into the cytosol of the cells. Ia ADP-ribosylates actin, resulting in cell rounding and death.
Structural and biochemical characterization of Clostridium perfringens ...
2023年4月18日 · The Gram-positive bacterium Clostridium perfringens has a major pilin, and a minor/tip pilin (CppB) with the collagen-binding motif. Here, we report X-ray structures of CppB collagen-binding domains, collagen-binding assays and mutagenesis analysis, demonstrating that CppB collagen-binding domains adopt an L-shaped structure in open form, and ...
Cryo-EM structures of Clostridium perfringens enterotoxin ... - Cell …
2024年11月7日 · Here, we present the structures of CpE in complex with its native claudin receptor in humans, claudin-4, using cryogenic electron microscopy. The structures reveal the architecture of the claudin/CpE complex, the residues used in binding, the orientation of CpE relative to the membrane, and CpE-induced changes to claudin-4.
X-ray structures of Clostridium perfringens sortase C with C …
2021年5月21日 · Here, we report biochemical analysis to detect the formation of Clostridium perfringens pili in vivo, and the X-ray structure of a novel intermolecular substrate-enzyme complex of CpSrtC with a sequence of LPST at the C-terminal site. The results showed that CpSrtC has a subsite for substrate-binding to aid polymerization of pili, and that the ...
Structural basis of Clostridium perfringens enterotoxin ... - Cell …
2024年2月8日 · Clostridium perfringens enterotoxin (CpE) is a β-pore forming toxin that disrupts gastrointestinal homeostasis in mammals by binding membrane protein receptors called claudins. Although structures of CpE fragments bound to claudins have been determined, the mechanisms that trigger CpE activation and oligomerization that lead to formation of ...
Clostridium perfringens - an overview | ScienceDirect Topics
Clostridium perfringens (formerly known as Clostridium welchii) is a Gram-positive, rod-shaped, anaerobic, spore-forming bacterium found as a normal component of soil, decaying vegetation, marine sediment, and the intestinal tract of animals including humans.
The pore structure of Clostridium perfringens epsilon toxin
2019年6月14日 · Here we show the cryo-electron microscopy structure of Etx pore assembled on the membrane of susceptible cells. The pore structure explains important mutant phenotypes and suggests that the...
Everything Illuminated— Clostridium perfringens β-toxin - Cell …
2020年7月8日 · In this issue of Cell Host & Microbe, Bruggisser et al. show that Clostridium perfringens β-toxin (CPB) binds platelet endothelial cell adhesion molecule-1 (PECAM-1 also known as CD31) to induce membrane pores. The discovery explains the cell type specificity for CPB and, in large part, the basic pathophysiology of disease.
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