网格蛋白依赖的胞吞及运输作用 - 知乎 - 知乎专栏
2023年4月11日 · 当配体与膜上受体结合后，网格蛋白聚集在膜下，逐渐形成直径50-100nm的质膜凹陷，称网格蛋白包被小窝(clathrin-coated pit)。 一种小分子GTP结合蛋白--发动蛋白（dynamin）在深陷的包被小窝的颈部组装成环，发动蛋白水解与其结合的GTP引起颈部缢缩，最 …
Mechanisms of clathrin-mediated endocytosis - Nature
2018年2月7日 · Clathrin-coated endocytic vesicles are produced by a complex modular protein machinery that transiently assembles on the plasma membrane. This machinery selects and concentrates cargo molecules...
Molecular mechanism and physiological functions of clathrin …
2011年7月22日 · Clathrin-coated vesicle formation proceeds through five stages that correspond to ultrastructural and cell biological observations: initiation, cargo selection, coat...
Molecular Structure, Function, and Dynamics of Clathrin …
Clathrin is a molecular scaffold for vesicular uptake of cargo at the plasma membrane, where its assembly into cage-like lattices underlies the clathrin-coated pits of classical endocytosis. This review describes the structures of clathrin, major ...
Clathrin coated pits, plaques and adhesion - ScienceDirect
2016年10月1日 · Clathrin coated pits are specialized patches at the plasma membrane that concentrate receptors, curve to form an invagination and bud off with their receptor cargo in the process of clathrin mediated endocytosis (CME) (Robinson, 2015).
Regulation of Clathrin-Mediated Endocytosis - PMC - PubMed …
CME occurs via the assembly and maturation of clathrin-coated pits that concentrate cargo as they invaginate and pinch off to form clathrin-coated vesicles. In addition to the major coat proteins, clathrin triskelia and adaptor protein complexes, CME requires a myriad of endocytic accessory proteins and phosphatidylinositol lipids.
Clathrin-Mediated Endocytosis - Madame Curie Bioscience …
We provide a mechanistic description of how a clathrin-coated vesicle (CCV) is formed, from the stages of initiation to scission to uncoating, as well as address important regulation by protein and lipid kinases and phosphatases.
Architecture of the AP2/clathrin coat on the membranes of clathrin ...
Here, we used cryo–electron microscopy, tomography, and subtomogram averaging to determine structures, interactions, and arrangements of clathrin and AP2 at the key steps of coat assembly, from AP2 in solution to membrane-assembled clathrin-coated vesicles (CCVs).
Clathrin light chain A drives selective myosin VI recruitment to ...
2019年10月31日 · The clathrin triskelia assemble around a budding vesicle, creating a characteristic polyhedral coat, forming a clathrin-coated pit (CCP) that captures protein cargo and further matures into a...
The First Five Seconds in the Life of a Clathrin-Coated Pit - Cell …
2012年8月3日 · Single-molecule studies reveal the first steps in the assembly of clathrin-coated pits, beginning with the coordinated arrival of a clathrin triskelion and two AP2 adaptor proteins. Whereas initial assembly requires PtdIns(4,5)P2, which mediates the association of AP2 with the membrane, sustained growth of the clathrin coat depends on the ...