DHα competent cells and Stellar Competent Cells were used for molecular cloning, while E.coli One-Shot BL21star (DE3 ... All the other mutants were obtained using In-Fusion®Snap Assembly protocol, ...
A recent protocol for PASTE (programmable addition via site-specific targeting elements) combines prime editing and site-specific integrases to integrate large genetic payloads. Relapse in T cell ...