The required temperature range was set from 4.0 ℃ to 98 ℃. P15 (300 nM) and template RNA (600 nM) were annealed in buffer at 25°C [50 mM HEPES, 20 mM NaCl, 1.0 mM KCl, 5 mM MgCl 2 and 4 mM DTT (pH 7.0 ...