Structure information is essential for understanding the function of a protein. For proteins that are difficult or impossible to crystallize NMR spectroscopy is the method available for 3D structure ...
Nuclear magnetic resonance (NMR) spectroscopy is a technique that detects the chemical environment of atomic nuclei by the absorption of radio-frequency electromagnetic radiation when in the ...
A variant of α-LA, in which all eight cysteines have been mutated to alanine (all-Ala α-LA), has been studied using NMR spectroscopy. At low pH all-Ala α-LA is nearly as compact as wild type α-LA.