She was using western blotting to study large ribosomal proteins, employing a protocol with two overnight steps. “Finally, I had that blot, and went to image it, but there was nothing there.

This procedure was named for its similarity to the previously invented method known as the Southern blot. The first step in a western blot is to prepare the protein sample by mixing it with a ...

Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. However, in recent years the reproducibility ...