But what is real time RT–PCR? How does it work? How is it different from PCR? And what does this have to do with nuclear technology? Here’s a handy overview of the technique, how it works and a few ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

A variety of probes specific for the amplified target (or amplicon) can be used in real-time PCR. By far the favorite, especially among scientists who have just started using the technique ...

As the expression level of a large number of genes can be roughly estimated with complementary DNA array techniques, quantitative RT-PCR provides a tool for closer scrutinization of the expression ...