Conventional targeted RNA-seq methods often require labor-intensive pre-sequencing enrichment steps, which can compromise comprehensive transcriptome profiling and limit their broader applications.

RNA sequencing analysis pipeline using STAR, RSEM, HISAT2 or Salmon with gene/isoform counts and extensive quality control.

enables precise multiomic characterization of biomolecular interaction networks at target RNAs. Distinct O-MAP workflows reveal RNA-adjacent proteins, transcripts and genomic loci.

Purple tea, the product of long-term natural evolution, stands out for its high anthocyanin content, known for antioxidant, ...

163.com Objectives The heterogeneity of meniscus cells and the mechanism of meniscus degeneration is not well understood. Here, single-cell RNA sequencing (scRNA-seq) was used to identify various ...

The analysis includes loading count data from an Excel file, differential expression analysis, generating a clustermap, performing principal component analysis (PCA), and creating a volcano plot.

Here, we report a systematic transcriptional atlas to delineate molecular and cellular heterogeneity in GA using single-cell RNA sequencing (scRNA-seq). Design We performed unbiased transcriptome-wide ...

In this work, we employ direct RNA sequencing with nanopores, enhanced by 5’ end adaptor ligation, to comprehensively interrogate the human transcriptome at single-molecule and nucleotide resolution.

In this valuable study, the authors used an elegant genetic approach to delete EED at the post-neural crest induction stage. The usage of the single-cell RNA-seq analysis method is extremely suitable ...

DNase is used to degrade DNA in samples where RNA purity is required, such as RNA extraction for RT or RNA sequencing. Similarly, RNase is employed to remove RNA in DNA-only assays, such as during ...

本文通过O-MAP（RNA接近标记）技术的实施，结合质谱（O-MAP-MS）、测序（O-MAP-seq）及染色质免疫沉淀（O-MAP-ChIP），揭示了RNA与其相互作用蛋白及转录组之间的精细关系。特别是，作者针对RNA介导的细胞隔室化现象，通过O-MAP技术的高灵敏度和空间精度，深入分析了 ...