自二十世纪九十年代中期以来，芯片就一直是基因组表达分析的中坚力量。不过随着测序成本的直线下降，RNA测序（RNA-seq）成为了越来越受欢迎的转录组分析方法。The Scientist杂志与多位专家共同探讨了从芯片到RNA-seq的过渡，希望帮助研究者们顺利度过这段艰难 ...

引言人类胚胎发育的过程犹如一幅精妙绝伦的生命画卷，从一个微小的受精卵开始，短短数周内通过复杂而精准的细胞分裂与分化，演变成具有初步结构和功能的胚胎。这一过程不仅展现了生物学的奥妙，也蕴藏着生命起源的答案。尽管人类对自身的了解日益深入，胚胎发育的早期阶 ...

日前，武汉大学博士毕业生、目前正在美国芝加哥大学从事博士后研究的吴晋军，和所在团队通过使用 ARTR-seq 这一转录组测序技术，让研究核斑点功能和 RNA 定位测定向前迈出了一步，也为揭示核斑点在 RNA ...

Conventional targeted RNA-seq methods often require labor-intensive pre-sequencing enrichment steps, which can compromise comprehensive transcriptome profiling and limit their broader applications.

Years of experiments and databases filled with RNA-seq results belie the simple reality that, until fairly recently, it was impossible to analyze RNA directly. The RNA-seq studies performed with ...

RNA sequencing is a powerful tool that helps researchers uncover novel biological insights into the transcriptome. This free eBook allows readers to learn about RNA sequencing (RNA-Seq ...

论文共同通讯作者、芝加哥大学生物化学与分子生物学教授Chuan He博士说，“一旦你看到这些 ...

The next step is very similar to RNA-Seq. RNC sequencing is a method for profiling the Ribosome Nascent-chain Complex using next-generation sequencing (NGS) (RNC). The ribosome attached to the ...

研究人员在恶性上皮细胞中鉴定出五种独特的表达程序，揭示了肿瘤内的异质性。他们还根据基因表达的差异将嗅神经母细胞瘤分为三种亚型：神经、基底和间质（neural、basal & mesenchymal）。其中，间质亚型是他们首次提出的。

Fritz Sedlazeck, Genome Research publishes a diverse collection of research and review articles highlighting novel ...