In this method, the downstream primer, in addition to the target-specific sequence contains a full consensus sequence, of viral RNA promoter, and the PCR product can be used directly as a template ...
In addition to the template, DNA polymerases need a pre-existing DNA or RNA chain end (a primer) onto which to add each nucleotide. For this reason, the lagging strand polymerase requires the ...
Comparison of a single-stranded RNA and a double-stranded DNA with their corresponding nucleobases. (Image: Wikimedia Commons, CC SA 3.0) The most common type of base pairing is the Watson-Crick base ...
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