Here, we used deep full-length single-cell RNA-sequencing to distinguish post-mitotic cone and rod developmental states and cone-specific features that contribute to retinoblastomagenesis. The ...

We lentivirally delivered the shRNA constructs into the cell lines and allowed them to grow for 10 cell divisions. As a baseline control, we harvested cells 24 h after library transfection. Assuming ...

In the quest to enhance the efficacy of chimeric antigen receptor T (CAR-T) cell therapy against hepatocellular carcinoma ...

Researchers uncover a link between impaired glutathione–ascorbate metabolism and early dopamine increases in Huntington's ...

Background: Dox cardiomyopathy remains a clinically significant problem. Alternate-day fasting has been shown to exacerbate Dox cardiomyopathy and cachexia. We sought to identify common disease ...

ROBO1-specific siRNAs were transfected in SGC7901-M cells. According to the western blot analysis, siRNA4 had the highest inhibitory rate and was selected to construct the lentiviral expression vector ...

The study commenced with the isolation and culture of primary human T lymphocytes, which were subsequently transduced with a ...

Glucagon-like peptide 1 (GLP-1) is a gut-derived hormone secreted by intestinal L cells and vital for postprandial glycemic control. As open-type enteroendocrine cells, whether L cells can sense ...

RT-PCR and/or western blot analyses were performed to confirm target knockdown by siRNA. Short hairpin RNAs (shRNAs) (Sigma-Aldrich) were used to stably infect NCI-N87 GC cells (shRNA empty vector or ...