Conditional alleles may be given priority when knockout alleles ... at the gene expression level. Finally, we note that introducing experimental envirotypes into mouse phenotyping protocols ...

to knockout and characterize all protein-coding genes in the mouse genome. Knocking out the activity of a gene provides valuable clues about what that gene normally does. However, creating and ...

Endocannabinoids, molecules naturally produced in the brain, play a critical role in regulating appetite, energy use, and ...

Figure 1: Delivery of synthetic shRNA into Nanog-GFP mouse ES cells. M15 ES cell medium with leukemia inhibitory factor (LIF): DMEM Knockout (Gibco), 15% fetal bovine serum (FBS; StemCell ...

The investigator will initiate a CRISPR-KI request in iLab. The request form requires the gene name and a brief allele description. An IACUC protocol number, mouse transfer information, and a charge ...

Researchers at Baylor College of Medicine, Jan and Dan Duncan Neurological Research Institute (Duncan NRI) at Texas ...

A research group has discovered that in mammals, a protein kinase A (PKA) promotes wakefulness, while protein phosphatase 1 (PP1) and calcineurin promote sleep. This study revealed that the balance ...

Both gene knockout and knock-in models are powerful tools widely used in biomedical research to gain important insights into ...

The study is published in Nature. Focusing on PKA and dephosphorylation enzymes, the research group created comprehensive gene knockout mice and conducted further experiments inducing the ...

While previous attempts have been made, in-depth phenotypic changes resulting from mitochondrial gene knockout ... within the cerebral cortex of these mice, accompanied by hippocampal atrophy ...

Researchers have uncovered how loss of the MeCP2 protein triggers early molecular changes leading to Rett syndrome, a severe ...