细胞内吞和细胞连接通透性是细胞生物学中的两个重要过程,对细胞的物质运输、信号传导和细胞间相互作用起着关键作用。FITC-Dextran因其分子大小和荧光特性,可以被用作探针来测量这两个过程。 细胞内吞: 原理:将FITC-Dextran与细胞共培养一段时间,细胞将 ...
原理:细胞间的连接结构(如紧密连接、间隙连接等)对物质的通透性具有调节作用。将FITC-Dextran施加到细胞外,通过观察其在细胞间的扩散情况来评估细胞连接的通透性。 应用:利用这一原理,可以研究不同细胞连接类型的通透性,以及在不同生理或病理条件 ...
在细胞生物学的研究领域,科学家们找到了一种创新的工具——葡聚糖偶联荧光素(Dextran4k-FITC),来深入探索细胞内吞和细胞连接通透性的奥秘。 Dextran4k-FITC,这一特殊的产品,以其独特的激发波长496nm和发射波长520nm,在荧光显微镜下呈现出明亮的绿色荧光。其分子量覆盖2k至2000k,为研究者提供了多种选择,以适应不同实验需求。 细胞内吞是细胞通过摄取外界物质来维持生命活 ...
Design Experiments were performed under basal and TGR5 agonist treatment. In vitro transepithelial electric resistance (TER) and FITC-dextran diffusion were measured in different cell lines. In vivo ...
This valuable study examines how different exercise training intensities affect intestinal barrier function and gut microbiota composition over a 6-week period in mice. The evidence supporting the ...
A test of HAEC cell membrane permeability using fluorescent intensity of FITC-Dextran (40kD) showed that Octacosanol can significantly improve cell integrity to damage induced by LPS at 100ng/ml for 4 ...
Get detailed information on Dextran, including pronunciation, uses, dosage guidelines, indications, and instructions on how and when to take it and when to avoid it. The updated prescription ...
Fresh HBSS was then added to both the apical and basolateral chambers. After 15 min of preincubation, HBSS in the apical and basolateral chambers was replaced with HBSS containing FITC-dextran 3–5 kDa ...