For library preparation, immunoprecipitated chromatin was eluted in elution buffer (1x TE, 250 mM NaCl and 0.3% SDS). Chromatin proteins and RNA were digested with Proteinase-K and RNase A ...
Chromatin organization strengthens DNA for mechanically stressful processes like mitosis, helps protect DNA from damage and is important in the control of gene expression and DNA replication.
is the most common approach to observe global binding of proteins to DNA in vivo. The occupancy of transcription factors (TFs) from ChIP-seq agrees well with an alternative method, chromatin ...
A multi-institutional team of researchers, led by Georgia Tech's Francesca Storici, has discovered a previously unknown role for RNA. Their insights could lead to improved treatments for diseases ...
The eukaryotic genome is organized in the form of a highly compact nucleoprotein complex, the chromatin. Nucleosome, the basic unit of chromatin, consists of ~147 bp of DNA wrapped 1.65 times around a ...
At the heart of molecular biology is the study of genetic material – DNA and RNA – which serve as the fundamental blueprints for life. These nucleic acids are not only essential for understanding how ...
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