This work will be of high interest to a broad swathe of scientists studying native macromolecules present at low concentrations in cells. Cryo-EM single-particle analyses typically require target ...
then discard the supernatant. Next, we used red blood cell lysate (10×) (Sigma-Aldrich, St. Louis, MO, USA) to remove red blood cells from the single-cell suspension. In short, add 1x Lysis Buffer to ...
Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Groningen 9713AV, The Netherlands ...