Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...

This classification is divided into two types: differential and density. Differential centrifuges are used to separate particles from a liquid medium or to separate particles of different masses into ...

Carefully remove the supernatant and discard it. Estimate the volume of the pellet, and resuspend it in five volumes of ice-cold PM buffer as in Step 6. Incubate on ice for 10 min. Centrifuge the ...

Cell debris and partially digested organelles etc. can be pelleted by centrifugation leaving the cell extract as a reasonably clear supernatant. 2. Purification of DNA from cell extract In addition to ...