Afterward, 30 μL supernatant was added into 200 μL 5 mM ammonium formate containing internal standards at indicated concentrations. Then, all the samples were vortexed for 30 s and centrifuged at ...
Overall, these results will be useful to the RNA community. The mRNA 5'-cap structure removal by the decapping enzyme DCP2 is a critical step in gene regulation. While DCP2 is the catalytic subunit in ...