The preparation may now be introduced into cells by microinjection and observed using fluorescence microscopy as described in ref. 12. Problem: The yield of labeled tubulin is low, or the dye to ...

These are immunofluorescence analysis of SH-SY5Y cells treated for 5 days with 10uM Retinoic Acid and 50ng/ml BDNF for the next 3 days. The DAPI fluorescence stain is blue and Beta-tubulin is green.