This protocol describes the first stages of Southern blotting: digestion of genomic DNA with restriction enzymes, separation of the resulting fragments by gel electrophoresis, and capillary ...

The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...

Southern blotting, restriction fragment length polymorphism (RFLP), and analysis via mass spectrometry. Before electrophoresis begins, a user prepares and cools a liquid gel, pours it into a tray, and ...

This method was named for its similarity to the technique known as a Southern ... called gel electrophoresis. Following separation, the RNA is transferred from the gel onto a blotting membrane.