This core performs non-radioactive, RNA in situ hybridization (ISH) on tissue sections. A unique high-throughput technology developed by the core (Yaylaoglu MB, Titmus A, Visel A, Alvarez-Bolado G, ...
All steps of ISH – from pre-hybridization to additions of probes, hybridization, stringency washes and antibody-mediated chromogenic or fluorescent reaction – are carried out by the robotic system.
(a) The basic elements of FISH are a DNA probe and a target sequence. (b) Before hybridization, the DNA probe is labeled by various means, such as nick translation, random primed labeling ...
The Karolinska Institutet team now believes that TRISCO may be suitable not only for studying intact mouse brains, but can be used for larger brains such as those of guinea pigs, as well as various ...