This powerful approach to cutting and pasting DNA molecules is known as DNA cloning or recombinant DNA technology. When they act on a DNA molecule, restriction enzymes produce "blunt" ends when ...

blunt and perhaps containing sites for restriction enzymes. 'Old-fashioned' cloning still works, and for years, researchers relied on using cloning methods in which vector and insert were ...

A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...