I therefore devised a single-tube, nested PCR method that is easy to handle and yields consistent results. The PCR mix contained 8 pmol of each reverse and FAM-M13(-21) primer and 2 pmol of the ...
You have full access to this article via your institution. To demonstrate the power of PCR–mediated recombination and provide an example of the general utility of the method, we constructed ...
Here’s a handy overview of the technique, how it works and a few refresher details on viruses and genetics. What is real time RT–PCR? Real time RT–PCR is a nuclear-derived method for detecting the ...