“My lab has been using PCR for more than two decades — initially for cancer testing, but in the past five years, we have seen a dramatic increase in PCR for microbiological testing,” he says.
Concerns were raised when people had positive lateral flow tests, but negative follow-up PCR results from the lab between 8 September and 12 October - most affected live in the South West of England.
Since March, 183,939 pupils in England have tested positive on a lateral flow test at home and then had a sample sent off to a laboratory for a follow-up PCR test. In total 89% of those swabs ...
The problem: Wakefield had incorrectly applied the common laboratory protocol known as quantitative real-time polymerase chain reaction (qPCR ... added to the test tube mix as a control.