However, under the low stringency conditions required for short nucleic acid probes to form stable interaction, the longer single-stranded targets are able to engage in secondary structure.
Improve assay sensitivity and specificity with Locked Nucleic Acid oligonucleotides (LNA oligos ... Additional advantages: Access LNA probes for multiplexing with a full suite of fluorophores.
Ten years ago, a pair of scientists described a new kind of probe for quantifying nucleic acids that could do it in real-time and in solution 1. No purification, no gels, no radioactivity (and no ...
Quantitative real-time PCR (qPCR) is the tool of choice for quantifying nucleic acids in a variety of research fields ranging from agricultural biology to molecular diagnostics. Because factors such ...