The technique described here, which uses gel filtration to separate high-molecular ... DNA is excluded and passes directly through the column. Bio-Gel P-2 can be used to separate oligonucleotides ...

Today, column-based separation generally has gone ... Nevertheless, traditional gel filtration remains popular for several applications—including the widely used method for the purification ...

For each purification step, one always must achieve a balance between high yield and highest increase in purity. For example, if you only have a 2 mL fraction of the gel filtration column, you would ...