Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...

The study of the cell cycle focuses on mechanisms that regulate the timing and frequency of DNA duplication and cell division. As a biological concept, the cell cycle is defined as the period ...

The primary G1/S cell cycle checkpoint regulates eukaryotic cells’ commitment to enter DNA synthesis (S phase) and serves as a stop/go decision prior to cell division. Cyclin-dependent kinase ...

Dr. Shweta Tyagi, Staff Scientist and Head of the Laboratory of Cell Cycle Regulation at CDFD, chaired the event and ...

When looking at cells with a microscope, the length of different stages of the cell cycle can be estimated using the formula: \(\text{Length of time in phase}=\) \(\frac{\text{observed number of ...

In the course of growth and division, eukaryotic cells duplicate their genomes with remarkable fidelity. The precision of this process depends in large measure upon stringent regulatory mechanisms ...

She and her team found that this effect happens through an unusual stress response. "If there is a problem with DNA packaging ...

She and her team found that this effect happens through an unusual stress response. "If there is a problem with DNA packaging, the cell quickly senses this issue. But instead of triggering a typical ...

POLK expression in neurons and report an important observation that POLK exhibits an age-dependent change in subcellular localization, from the nucleus in young tissue to the cytoplasm in old tissue.