Manipulations and transport to the centrifuge should be done on ice. 2| Discard the supernatant. Wash the cell pellet with 10 ml of ice-cold 1× phosphate-buffered saline. Pellet cells at 300g at ...

Carefully remove the supernatant and discard it. Estimate the volume of the pellet, and resuspend it in five volumes of ice-cold PM buffer as in Step 6. Incubate on ice for 10 min. Centrifuge the ...